P.N. Navya, H.K. Daima
siddaganga institute of technology,
Keywords: isoniazid, gold, silver nano-systems, antibiotic corona, metal core, biological events
Summary:Innovative approaches to engineer nanoparticles with desired physicochemical characteristics open new opportunities to utilise such materials for assorted applications. In this context, various methods have been developed to prepare metal nanoparticles. However, most of the metal nanoparticles synthesis routes have primarily used chemicals for their synthesis and stability, which is major limiting factor for these materials in biological applications. Such chemical based synthesised nanoparticles may have potential unwanted toxic impact to biological entity. In the present work, we have established a single step distinctive synthesis method to obtain monometallic gold (Au), silver (Ag) and bimetallic Au-Ag nanoparticles systems with control over composition. Moreover, antibiotic surface corona on these nano systems was developed by employing isoniazid as reducing and stabilizing agent. Isoniazid is first-line antibiotic agent, which is used to prevent and treat both the latent and active tuberculosis infections. The formation of Au, Ag and Au-Ag nano-systems, their composition and surface chemistry was established by UV-vis, DLS, Zeta potential and FTIR measurements. The presence of isoniazid corona on Au and Ag based nano-systems can provide biological identity to these metal particles, which may be suitable to recognise mycobacteria, particularly Mycobacterium tuberculosis. Furthermore, due to the occurrence of organic surface corona of antibiotic, these nano-systems may act like natural enzymes. To validate our hypothesis, currently we are conducting in-vitro studies on these Au and Ag based nano-systems to scrutinize their peroxidase and catalase like behaviour. Additional experiments are being conducted to evaluate anti Mycobacterium tuberculosis potential of these nano-systems. Outcomes of this study have potential to open up new prospects toward careful design of antibiotic-functionalized metallic nano-systems, which will have significant potential to be used as new class of materials for variety of biological applications.