C.F. Rediguieri, M.H.A. Zanin, N.N.P. Cerize, A.M. Oliveira, T.J.A. Pinto
University of São Paulo,
Keywords: nanofibres, PCL, pluronic, tissue engineering, regenerative medicine
Summary:Since world population is ageing, regenerative medicine has become a growing area in the medical field in order to maintain the life quality of elderly population. Instead of repairing or transplanting a tissue or organ, scientists believe tissue engineering is a better idea to bring back the original functions of the body. Nanofibers have the potential to mimic the human tissue architecture at the nanometer scale, especially due to their large surface area and high porosity. PCL is a common polymer used in the fabrication of nanofibers scaffolds; however, its hydrophobic properties can hinder cell adhesion, and, consequently, cell proliferation. Pluronic® F-127 is a non-ionic hydrophilic copolymer used in cell culture applications. Therefore, the aim of this study was to develop a nanofibrous scaffold joining the thermal and mechanical properties of PCL and the cell-attractive properties of Pluronic. SEM, FTIR, DSC/TG, contact angle, and mechanical properties of scaffolds were assessed. Results revealed that the addition of small amounts of Pluronic® F-127 to PCL highly increased hydrophilicity of the scaffold, while maintaining the desired bulk (thermal and mechanical) properties of PCL. References: Cho, W. J., Kim, J. H., Oh, S. H., Nam, H. H., Kim, J. M., & Lee, J. H. (2009). Hydrophilized polycaprolactone nanofiber mesh-embedded poly(glycolic-co-lactic acid) membrane for effective guided bone regeneration. Journal of Biomedical Materials Research. Part A, 91(2), 400–7. Fu, S.-Z., Meng, X.-H., Fan, J., Yang, L.-L., Lin, S., Wen, Q.-L., … Chen, Y. (2014). In vitro and in vivo degradation behavior of n-HA/PCL-Pluronic-PCL polyurethane composites. Journal of Biomedical Materials Research. Part A, 102(2), 479–86. Kim, D. H., Heo, S.-J., Shin, J.-W., Mun, C. W., Park, K. M., Park, K. D., & Jee, K. S. (2010). Preparation of thermosensitive gelatin-pluronic copolymer for cartilage tissue engineering. Macromolecular Research, 18(4), 387–391. Lee, S. J., Oh, S. H., Liu, J., Soker, S., Atala, A., & Yoo, J. J. (2008). The use of thermal treatments to enhance the mechanical properties of electrospun poly(epsilon-caprolactone) scaffolds. Biomaterials, 29(10), 1422–30. Lippens, E., Vertenten, G., Gironès, J., Declercq, H., Saunders, J., Luyten, J., … Cornelissen, M. (2010). Evaluation of bone regeneration with an injectable, in situ polymerizable Pluronic F127 hydrogel derivative combined with autologous mesenchymal stem cells in a goat tibia defect model. Tissue Engineering. Part A, 16(2), 617–627. Oh, S. H., Kim, J. H., Song, K. S., Jeon, B. H., Yoon, J. H., Seo, T. B., … Lee, J. H. (2008). Peripheral nerve regeneration within an asymmetrically porous PLGA/Pluronic F127 nerve guide conduit. Biomaterials, 29(11), 1601–9. Pan, J., Liu, N., Sun, H., & Xu, F. (2014). Preparation and characterization of electrospun PLCL/Poloxamer nanofibers and dextran/gelatin hydrogels for skin tissue engineering. PloS One, 9(11), e112885. Vasita, R., Mani, G., Agrawal, C. M., & Katti, D. S. (2010). Surface hydrophilization of electrospun PLGA micro-/nano-fibers by blending with Pluronic?? F-108. Polymer, 51(16), 3706–3714.