P. Cortes, D. Fagan, A. Olszewki, M. Radetic, G. Abdallah
Youngstown State University,
Keywords: CNT, phage display library, bio-sensors
Summary:The present work has studied the selectivity process of a biological complex based on Carbon Nanotubes and a specific amino-acid sequence for interacting specifically with blood. In this work, Human Serum Albumin (HSA) has been targeted as an indicator of blood, and a short chain peptide, GAQGHTVEK (GK-1) has been used as the biological system for binding the HSA. Here, the peptide has shown to specifically bind to serum albumin (at least six times) over a wide range of bodily fluids and everyday liquid media such as urine, water, juice, and coffee. In this research program, the protein has been covalently attached to the surface of COOH- functionalized multi-wall carbon nanotubes through a carbodiimide catalyzed amide (EDC) linkage, and the binding of HSA by the MWCNT-GK-1 complex has been confirmed. The selectivity of the investigated semi-conductor nano-bio complex, can serve as a technical platform for developing biomarkers, or bio-sensing textiles where, the CNT-protein complex can act as the stable and robust solid-state detecting core material.