J.W. Gillespie and V.A. Petrenko
Keywords: phage display, targeted drug delivery, nanomedicines
Summary:Analysis of multi-thousand clone populations of the cancer cell-binding landscape phages and their multi-motif proteins using massively parallel sequencing techniques allowed the discovering of a variety of short motifs serving as elementary binding units during phage selection—a ‘molecular LEGO’ for engineering of proteins with predicted cell binding, penetrating and migrating performance. This finding inspired us to propose a novel “addressed drug navigation” concept, which relies on the use of “self-navigating ligands”, selected from polyvalent landscape phage display libraries and accumulating EBUs responsible for binding to different tissue cells. Applied to the targeted drug delivery problem, this novel approach promises to replace the existing ‘point to point’ targeting concept for the novel ‘self-navigating’ drug delivery paradigm that can be used as a theoretical basis in development of a novel generation of molecular imaging probes and medications for precise and personal medicine.