Fluorescence Lifetime Trajectory of the Mouse Pre-Implantation Embryopredicts its Viability

N. Ma, N.R. de Mochel, T.Y. Yoo, P.D.A. Pham, K. Cho, M.A. Digman
University of California, Irvine,
United States

Keywords: non-invasive, imaging

Summary:

Here we describe the application of a non-invasive live imaging approach capable of measuring NADH levels within living mouse embryos undergoing in vitro culturing. We apply a phasor-FLIM (Fluorescence-Lifetime Imaging Microscopy) approach and capture information on the metabolic energy sources (e.g., NADH) utilized by pre-implantation embryos.