Keywords: biosensor, quartz, coagulation
Summary:Quartz resonator sensors can be used to measure changes in fluid viscosity or mass coupled to their surfaces (both of which occur during coagulation of blood) by monitoring the associated changes in the resonance frequency. The simple and robust sensing mechanism of these biosensors, combined with the capability of sensing biological processes, makes them promising point-of-care tools. However, despite many studies with these sensors, quartz resonator based POC sensors have not found widespread use due to the high liquid volume (>100 microliters), specific liquid chamber, and restrictive temperature control requirements. Here, we present microfluidics integrated quartz resonators that have extremely low liquid volume requirements (~10 nanoliters), does not require any liquid chambers, and can provide sensitive fluid properties characterization inside the microfluidics within seconds. With these capabilities, we are working towards creating a point-of-care blood-plasma coagulation monitor that has on-chip blood plasma separation for more accurate coagulation measurements.