Oregon State University, Corvallis, OR, US
Ideal bioorthogonal reactions containing high reaction rates, high selectivity and high stability would allow for stoichiometric labeling of biomolecules in minutes and eliminate the need to washout excess labeling reagent. Currently no general method exists for controlled stoichiometric or sub-stoichiometric labeling of proteins in live cells. To overcome this limitation we have developed a faster more stable tetrazine-containing amino acid (Tet-2.0) and have genetically encoded this amino acid in response to an amber codon. We have demonstrated that in vivo reactions between protein containing Tet-2.0 and sTCO react with a biomolecular rate constant of 87,000±1440 M-1s-1. This bioorthogonal reaction is fast enough in cells containing Tet-v2.0-protein with sub-stoichiometric amounts of sTCO-label to remove the labeling reagent from media in minutes thereby eliminating the need to washout label. This ideal bioorthogonal reaction will enable the monitoring a larger window of cellular processes in real time.
Primary Application Area: Biotech, Pharma, Medical Devices
Technology Development Status: Prototype
Technology Readiness Level: TRL 4
Organization Type: Academic/Gov Lab
Showcase Booth #: 622
GOVT/EXTERNAL FUNDING SOURCES