Engineered Phage Matrix Stiffness-Modulating Osteogenic Differentiation

J-I Kang, S.Y. Yoo
Pusan National University,

Keywords: engineered phage, stiffness, osteogenic differentiation


Although Biochemical cues in extracellular matrices plays a critical role in regulating cellular growth and faith, their physical cues as stiffness in guiding stem cells has not been well studied so far. Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by crosslinking the engineered phage displaying RGD and HPQ with various concentrations of streptavidin or polymer, PDDA. Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than softer one. Our phage matrices can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation. [This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (H16C1067)]