Angio-PhaGel: Phage based Angiogenic Extracellular Matrix Niche Engineering

K. Raj Shrestha, J-I Kang, D.H. Lee, B.Y. Lee, S.Y. Yoo
Pusan National University,

Keywords: angio-phagel, phage engineering, angiogenic niche, extracellular materix


Although stem cell niche plays a vital role in stem cell differentiation towards different lineages, an artificial stem cell niche achieved so far is not successful to fulfill the complex microenvironment of the stem cell. Here, we demonstrated engineered hybrid phage matrices that possess cell adhesive and angiogenic peptides with a suitable scaffold by formulating polyacrylamide hydrogel incorporating phage in different stiffness to guide adult stem cells (ASC) and could achieve higher stiffness favoring osteogenesis and lower stiffness favoring adipogenesis. In this study, we present a specific phage based angiogenic matrices by modulating physical and biochemical cues in differentiation of ASC, providing convenient artificial stem cell niche. [This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (H16C1067)]