S.W. Leung, J.C.K. Lai
Idaho State University,
Keywords: metabolites, diagnosis, nanoparticles, biosensor, environmental monitoring
Summary:We have mounted 5 different biocatalysts on a sensor platform to examine the performance of this electrochemical sensing system for the detection of different biomolecules/metabolites and environmental important molecules, with such we also compared how this sensing system fares with literature results of similar measurements. The biocatalysts are LDH (lactate dehydrogenase), GDH (glutamate dehydrogenase), human IgG (human immunoglobulin G), Hb (hemoglobin), and PSA (prostate-specific antigen). The sensor platform constitutes of a layer of biocomposite mounted on different electrodes made out of Au, Ag, Pt, and glass carbon; the biocomposite is fabricated with polymers and sol-gel Au nanoparticles with or without an extra layer of biomolecules. The targeting species for measurements include NH4+, NO3-, CN-, H2O2, and the biomolecules specific to the biomolecules/antigens as mentioned above coated on the surface of the biocomposites. In this report, we will provide a systematic update of analyses of this sensing system, including the unique identification potentials and sensitivities. This novel sensing system can be a valuable tool in biomedical diagnosis and environmental forensics; in particular the sensor platform, any biomedical diagnosis can be conducted with extremely high sensitivity as long as the biomolecules and their antigens are known.