Biomimetic sensing of progesterone in pharmaceutical samples

A. Laza, A. Godoy, S. Pereira, P.R. Aranda, G.A. Messina, C.D. Garcia, F.A. Bertolino, J. Raba
Universidad Nacional de San Luis,

Keywords: biomimetic sensor, molecularly imprinted polymer, electropolymerization, progesterone


The developed biomimetic sensor for the determination of P4 was developed in a very simple process with great selectivity. This sensor presents competitive characteristics with biosensors and great chemical stability, being possible to use it several times while maintaining good response. It. presented good analytical characteristics for the determination of P4 with a linear response range from 0.75 to 45 µM with detection and determination limits of 0.12 µM and 0.41 µM, respectively. The sensor's selectivity was studied with compounds with very similar structure to P4 and no interference effects were observed. Regarding to the stability the sensor could be used even 30 days after its preparation and it is reusable at least 12 times maintaining a good response. The design and construction of the sensor is very simple and fast, achieving an electrochemical sensor with great selectivity, reproducibility (RSD=2.3%) and stability. The sensor was successfully used in the determination of P4 in pharmaceutical formulations.