J. Chang, M. Grillo, N. Barakat, J.J. Hickman
University of Central Florida,
United States
Summary:
With the utilization of primary human cells, Multi-Organ Physiological Systems (MPS) focus on providing a new method of drug discovery by analyzing acute and chronic effects of drug dosing measured on microelectrode arrays. Currently, studies of chronic dosing with MPS have been limited with cells having short adhesion times allowing only for acute studies to be effectively done Studies have shown, physical properties, such as surface roughness, within a body are variable and can outline how mirrored surfaces roughness and protein type can enhance adhesion of cells for a longer period of time. In this study, we are using acid-etched glass substrates with four different surface roughness values coated with laminin or collagen. The substrates will also be coated with diethylenetriamine (DETA), an amino-based silane which has been shown, through multiple studies, to improve cell adhesion. The conditions of the substrates will be monitored and fluorescently labeled for intensity changes over a period of 45 days. The necessity of determining optimal physical surface parameters for these micro physiological systems will aid in mimicking in vivo conditions to prolong cell adhesion for chronic studies.