B.A. Suleiman, Y.A. Suleiman
University of South Florida,
Summary:In recent years, gene delivery has emerged as a promising approach for the treatment of various genetic disorders. However, the success of gene therapy heavily depends on the efficient delivery of therapeutic genes to the target cells. Lipid nanoparticles (LNPs) have gained significant attention as a delivery system for gene therapy due to their biocompatibility, stability, and ability to protect nucleic acids from degradation. The synthesis of LNPs can significantly impact their properties, such as size, surface charge, and encapsulation efficiency. This review article aims to provide a comprehensive analysis of recent research on different lipid nanoparticle synthesis methods for gene delivery in pharmaceutical nanotechnology. In addition to comparing the various methods, this review will also examine the utility of these LNPs for gene delivery. The article will explore the factors that affect the efficiency of gene delivery, such as cellular uptake, endosomal escape, and gene expression. Furthermore, the review will also discuss the challenges and future directions in the field of LNP-based gene therapy.