M.C.S. Silva, M.C. Santos, M.V. Santana, F.R. Marciano, A.O. Lobo
Federal University of Piauí,
Brazil
Keywords: 3D bioprinting, hydrogels, oxygen-generating microparticles, vascularizatio
Summary:
To date the application of soft hydrogels to bone and cartilage regeneration is limited due to low mechanical modules (fracture and stiffness). To solve this, cryo(bio)printing surges as an alternative to improve the crystallinity of cross-linked hydrogels. Recently we developed a strategy to combine electrospun and rotary-jet spun ultrathin fibers and 3D bioprinted hydrogels to produce scaffolds for bone and cartilage tissue engineering. Firstly, ultrathin PCL and PLA fibers were produced using homemade electrospinning and rotary-jet spinning apparatus. Herein, we have characterized the hydrogels and ultrathin produced fibers using . Then, we have incorporated different layers into 3D bioprinted (37oC) and cryo(bio)printed (-20oC) GelatinMethacryloyl/Gelatin/Laponite hydrogels structures. Mesenchymal stem and chondrocytes were used and the cytotoxicity and live/dead cells were evaluated. The mechanical properties of produced were evaluated the engineering of oxygen-generating micro/nanoparticles in cells-laden bioinks through a combination of gelatin-methacryloyl (GelMA), gelatin and laponite. Different layers containing or non-micro/nanofibers were also evaluated looking for improve of mechanical properties. The hydrogels combined GelMA, gelatin and laponite displaying tunable rheological and mechanical properties after nanofibers mats incorporation (special after three layers), for sustained and prolonged oxygen-release. All biological assays will be discussed and compared to other hypoxic hydrogels constructions reinforced with nanofibers mats.