S. Mesiya, M.B. Vaughan, R. Wolf, M. Khandaker
University of Central Oklahoma,
United States
Keywords: nanofiber, gauze, wound healing, antimicrobial agent
Summary:
The goal of this study is to evaluate the in vivo cytotoxicity of Polycaprolactone (PCL) nanofiber bandage containing antibacterial agents. Twelve adults inbred Wistar rats, were divided into control and test animal groups. The control group received only wound, where the test rats received rat fibroblast seeded skin graft and antibacterial PCL bandage on the created wound. The wound area images were captured after 1, 3, 5, 12 and 28 days. Both control and test rats were euthanized after 28 days. Control and test rats in this study remained in good health and did not show any recovery complications after surgery. At retrieval, no visual signs of inflammatory or adverse tissue reactions were observed on the skin of control and test animals. Image analysis measured the percentage of wound healing contraction area with respect to surgery wound area for the control and test animal. The percentage of contraction area was found to be higher for PCL grafted rats (test group) compared to wound without graft (control). Currently, we are conducting the in vivo toxicity analysis from the collect blood, liver, and kidney samples using proteomics platform.