R. Egan
Johns Hopkins University Applied Physics Laboratory (JHU/APL),
United States
Keywords: Biomanufacturing, Biomolecular Engineering, Synthetic Biology
Summary:
Downstream purification remains a major bottleneck in chemical and biomanufacturing, constrained by chromatography’s low resin utilization, high buffer consumption, and limited scalability. While technical innovations in chromatography promise improved efficiency and consistency, their adoption is hindered by challenges including process complexity, control of transient states, sensitivity to feed variability, and integration with upstream and downstream unit operations. This effort describes recent progress in overcoming these challenges, and highlights system-level considerations necessary to enable robust, flexible chromatographic purification.